According to cancer study, the researchers hypothesizes that the consumption of epigallocatechin-3 gallate or EGCG found in green tea can inhibit the activation of IBC cell growth, invasive phenotype, and survival of SUM-149 and UM-190 cells. With EGCG’s ability, its effectiveness can suppress the progression of breast cancer cells and lymphangiogenesis production.
The control group was treated with doses of EGCG, or with the carrier DMSO, which refers to dimethyl sulfoxide, a dose that treats skin inflammation and other diseases because of its ability to reduce progression of cancer, such as breast cancer (Joe Bowman, 2016). In addition, the highest dose of EGCG used during the treatment. The reason of using DMSO as treatment to the control group is because according to cancer studies, over the properties of DMSO, it’s discussed that researchers reported “on a series of experiments on cancerous animals that discovered DMSO targets cancer cells while leaving healthy cells alone” (Paul Fassa, 2014); helping to differentiate between cancerous and noncancerous cells through the DMSO capacity, that way researchers can call out which is cancerous and what’s not.
The treatment that’s used on the experimental group was doses of EGCG of 5, 10, 20, 40, 60, 80 or 160 µm (Cell Culture and Treatment Conditions, 2013) to function with the doses of 10.9, 21.8, 43.6, 87.3, 130.9, 174.5, and 349.0 µM. The doses was used in the experimental group because it’s necessary to test out the effectuality of the EGCG coated on the breast cancer cells (IBC) from extremely low to its highest rate. Instead of doing the experiment right off the bat, it’s important to follow the procedure to the results.
In the Results section of the cancer study, it is reported that the EGCG demonstrated the inhibition of cell growth and the lymphangiogenesis production of pre-existing tumors “derived from SUM-149 IBC ALDH-positive stem-like cells in an orthotopic mouse model” (Mineva ND, Paulson KE, Naber SP, Yee AS, Sonenshein GE, 2013). Additionally, the detection of cancer decrease was made, which means that the treatment of EGCG starts to work immediately; after the detection of the cancer reduction, the researchers then, investigated the effects of EGCG on pre-existing IBC cells. As written in the journal section “EGCG Reduces Growth of Pre-existing Tumors Derived from SUM-149 Stem-like Cells”, in investigating the effects of EGCG on growth of IBC tumors, “ALDH-positive SUM-149 cells were injected in the mammary fat pad of a female mice” (Mineva ND, Paulson KE, Naber SP, Yee AS, Sonenshein GE, 2013). The specific result that the researchers reported is that the “palpable tumors” were detected in all mices. However, once the mices are treated with EGCG, a significant decrease in tumor cells was detected after 32 days.
The researchers did follow the scientific method in their experimental design. In the introduction, the researchers began with an observation before proposing their hypothesis. The hypothesis was then, tested further for observations and experiments. After testing the hypothesis, an analysis and date were made, which leads to overall results and conclusions that’s based on the hypothesis, whether it is supported or not. Overall, the scientific method is followed perfectly to form an exceptional report on EGCG and IBC.
The hypothesis is supportive to the experiment since there’s a possibility that green tea can restrict the growth of cancer cells. To conclude this experiment, it demonstrates the example of how the scientific method functions in an experiment and how valuable it is when using it to make scientific discoveries. After careful observations and following the procedure, it introduced me to the fact that green tea plays a medical role in breast cancer cells.

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